The collision involved a HGV and a VW Passat blue saloon.
This occurred at about 1am.
Two people, a man and a woman, have sustained serious injuries and remain in hospital.
Highways England said the northbound carriageway near Long Bennington was blocked after the collision.
A police spokesman said: "Two men and a woman sustained serious injuries and remain in hospital.
"Could any person who witnessed the collision and or has dash cam footage of the incident please ring 101 and quote incident number 23 of 14/4/19."
The road was closed between A52, Barrowby and A46, Newark junctions.
The northbound carriageway remains closed - 9.30am Sunday, April 14.