Verity Lee-Varley, Chloe Hind, Connie Emery and Richard Harvey sang together for the first time in the touching tribute - which was released as people clapped for the NHS on Thursday.
A stirring video shows them all singing their parts while cutting to poignant images of NHS and key workers during the coronavirus pandemic.
James Clarke, 25, who has organised the annual Worksop’s Got Talent since it began in 2016, said he came up with the idea as thank you to key workers.
Speaking about the former Worksop’s Got Talent winners he said: “I just thought it would be nice to show all four of them together - I’m proud of what the show’s achieved.”
Since the talent show’s inception event planner James has brought some big names to judge the contest at North Notts Arena.
They have included stars from Coronation Street, Britain’s Got Talent, Love Island, The Apprentice and Sheffield Wednesday.
Proceeds from the event - over £37,000 so far - go to blindness charity Retina UK.
The charity is trying to cure a rare eye condition James suffers from called retinitis pigmentosa - from which he will lose his sight if it does not succeed.
James said: “I’m really happy with the video and I love the song - it’s such a positive tune.”
He hopes to get all four singers together again for Worksop’s Got Talent 2020 - scheduled for November 27.
However he added: “I’m really nervous because no-one knows what the social distancing rules will be at that time.
“It’s such a big event now - we have over 700 people turn up every year.
“It’s become bigger than I ever thought it would be and it just gets bigger and bigger every year.
“I want to get the total up to £50,000 this year - if it happens.”