Fire crews from Maltby, Edlington and the technical rescue unit attended the scene at around 2.15am.
It is thought the collision involved two vans.
A spokesman for South Yorkshire Fire and Rescue Service said crews had to cut a 44-year-old driver of one van from his vehicle. He was taken to Rotherham Hospital with head, abdomen and leg injuries.
A passenger was taken to Doncaster Hospital with a suspected broken wrist.
Diversions are in place and the motorway is expected to re-open shortly.