Matlock Town player Jordan Sinnott, 25, was found with a fractured skull in Market Place, Retford, Nottinghamshire, on Saturday morning, January 25.
Appearing before Mansfield Magistrates’ Court on Wednesday, January 29, Cameran Matthews, Kai Donovan and Sean Nicholson confirmed their names but made no plea before District Judge Jonathan Taaffe.
Matthews, 21, of Denman Close in Retford and Denovan, 22, of Collins Walk in Retford, have been charged with manslaughter, affray and common assault. Nicholson, 21, of Beechways in Retford, has been charged with affray.
The court was told that trouble had started between the three and a group including Mr Sinnott, in the Vine public house, before spilling out into Market Place, where the alleged incident took place.
Mr Sinnott was seen to fall to the ground, but briefly get back to his feet before collapsing in a shop doorway, the court was told.
Family wept as Matthews and Nicholson were both denied bail by Judge Taaffe following appeals from their respective solicitors, while no application for bail was made for Donovan.
All three were remanded into custody to next appear at Nottingham Crown Court on Wednesday, February 26.
Mr Sinnott, who was on loan at Matlock Town from Alfreton Town, died in hospital shortly before 6pm on Saturday.
Starting his career with Huddersfield Town, he had also had a spell on loan with Chesterfield FC.
Detective Chief Inspector Rob Routledge, who is leading the investigation, said: “I would like to thank the public again for their response to our appeals, and I continue to encourage anyone who saw anything on Saturday night to come forward. Even the smallest detail could be vital to our investigation, so please speak to us if you know something about what happened.”
Anyone who has information or video footage is asked to call Nottinghamshire Police on 101, or Crimestoppers on 0800 555 111.