Public Health England figures show that 55,521 people had been confirmed as testing positive for Covid-19 by 9am on Wednesday (April 28) in Nottinghamshire up from 55,498 the same time on Tuesday.
The health body recently changed the way it records the figures.
Cases identified through a positive lateral flow test – those which can deliver results within half an hour – will no longer be counted if the person then takes a PCR test, which is sent to a lab to be checked, and receives a negative result within three days.
The rate of infection in Nottinghamshire now stands at 6,704 cases per 100,000 people, lower than the England average of 6,848.
Across the UK, the number of recorded cases increased by 2,166 over the period, to 4,411,797.
Nottinghamshire's cases were among the 328,597 recorded across the East Midlands, a figure which rose by 166 over the period.
Cumulative case counts include patients who are currently unwell, have recovered and those that have died.